This book provides up-to-date information on a wide range of developments in liquid, gel permeation and hydrophobic interaction chromatographic methods and applications. It presents cutting-edge reviews in the fields of bio-, analytical, organic, polymer, and pharmaceutical chemistry.
Contributors
Contents of Other Volumes
Chapter 1
Roles of High-Performance Liquid Chromatography in Nuclear Medicine
Steven How-Yan Wong
I. Introduction
II. Characteristics of Radiopharmaceuticals
III. Instrumentation
IV. Preparation and Purification
V. Analysis of ⁹⁹ᵐTc-Labeled Pharmaceuticals
VI. Conclusion
Acknowledgements
References
Chapter 2
Calibration of Separation Systems in Gel Permeation Chromatography for Polymer Characterization
Josef Janca
I Introduction
II. Calibration Using Standards with Narrow Distributions
III. Calibration Using Standards with Broad Distribution
IV. Universal Calibration
V. Special Procedures of Calibration
VI. Influence of Operational Variables on Results of Calibration
VII. Conclusions
Acknowledgements
Appendix: Applications of Universal Calibration Principles in Synthetic Polymer Characterization
References
Chapter 3
Isomer-Specific Assay of 2, 4-D Herbicide Products
By HPLC: Regulatory Methodology
Timothy S. Stevens
I. Introduction
II. Ion-Exchange Chromatography Versus Revers-Phase Chromatography
III. Method Criteria
IV. Impurities in 2, 4-D
V. Separation of Impurities
VI. Alternative Columns
VII. Column Stability
VIII. Precision and Linearity
IX. Mobile Phase Modifier
X. Conclusions
References
Chapter 4
Hydrophobic Interaction Chromatography
Stellan Hjerten
I. Introduction
II. Definition of Hydrophobic Interaction and the Structure of Water
III. Changes in the Water Structure upon Solubilization of a Nonpolar Compound in Water
IV. Chemical Nature of the Hydrophobic Interaction
V. Charged Amphiphilic Adsorbents
VI. Neutral Amphiphilic Adsorbents
VII. Multipoint Interaction
VIII. Establishment of Appropriate Experimental Conditions for a Run
IX. Applications
Acknowledgements
References
Chapter 5
Liquid Chromatography with Programmed Composition of the Mobile Phase
Pavel Jandera and Jaroslav Churacek
I. Introduction
II. Retention Characteristics in Isocratic-Elution Chromatography and in Chromatography with Programmed Composition of the Mobile Phase
III. Practical Aspects of Chromatography with Programmed Composition of the Mobile Phase
IV. Conclusions
V. Summary
Appendix A: List of Symbols Used
References
Chapter 6
Chromatographic Separation of Aldosterone and its Metabolites
David J. Morris and Ritsuko Tsai
I. Introduction
II. Metabolism and Mechanism of Action of Aldosterone
III. Separation and Column Chromatography of Reduced and Polar Aldosterone Metabolites
IV. High-Pressure Liquid Chromatography of Reduced and Polar Aldosterone Metabolites
V. Improved Separations of Aldosterone Metabolites Using High-Pressure Liquid Chromatography
VI. High-Pressure Liquid Chromatography of Aldosterone of Aldosterone Metabolites Synthesized in Vitro
VII. Aldosterone Metabolites in Male Rat Urine
VIII. Conclusions
Acknowledgments
References
Author Index
Subject Index
Biography
J. Calvin Giddings (University of Utah, Salt Lake City, USA)