With a history that likely dates back to the dawn of human civilization more than 10,000 years ago, and a record that includes the domestication and selective breeding of plants and animals, the harnessing of fermentation process for bread, cheese, and brewage production, and the development of vaccines against infectious diseases, biotechnology has acquired a molecular focus during the 20th century, particularly following the resolution of DNA double helix in 1953, and the publication of DNA cloning protocol in 1973, and transformed our concepts and practices in disease diagnosis, treatment and prevention, pharmaceutical and industrial manufacturing, animal and plant industry, and food processing.
While molecular biotechnology offers unlimited opportunities for improving human health and well-being, animal welfare, agricultural innovation and environmental conservation, a dearth of high quality books that have the clarity of laboratory manuals without distractive procedural details and the thoroughness of well-conversed textbooks appears to dampen the enthusiasm of aspiring students. In attempt to fill this glaring gap, Handbook of Molecular Biotechnology includes four sections, with the first three presenting in-depth coverage on DNA, RNA and protein technologies, and the fourth highlighting their utility in biotechnology. Recognizing the importance of logical reasoning and experimental verification over direct observation and simple description in biotechnological research and development, the Introduction provides pertinent discussions on key strategies (i.e., be first, be better, and be different), effective thinking (lateral, parallel, causal, reverse, and random), and experimental execution, which have proven invaluable in helping advance research projects, evaluate and prepare research reports, and enhance other scientific endeavors.
Key features
- Presents state-of-the-art reviews on DNA, RNA and protein technologies and their biotechnological applications
- Discusses key strategies, effective thinking, and experimental execution for scientific research and development
- Fills the gap left by detailed-ridden laboratory manuals and insight-lacking standard textbooks
- Includes expert contributions from international scientists at the forefront of molecular biotechnology research and development
Written by international scientists at the forefront of molecular biotechnology research and development, chapters in this volume cover the histories, principles, and applications of individual techniques/technologies, and constitute stand-alone, yet interlinked lectures that strive to educate as well as to entertain. Besides providing an informative textbook for tertiary students in molecular biotechnology and related fields, this volume serves as an indispensable roadmap for novice scientists in their efforts to acquire innovative skills and establish solid track records in molecular biotechnology, and offers a contemporary reference for scholars, educators, and policymakers wishing to keep in touch with recent developments in molecular biotechnology.
Chapter 1 Molecular biotechnology: from past achievements to future breakthroughs
(Dongyou Liu)
Section I DNA Technologies
Chapter 2 DNA structure and function
(Dongyou Liu)
Chapter 3 DNA purification
(Na Li, and Dongyou Liu)
Chapter 4 DNA amplification
(Wenbao Zhang, Jun Li, and Dongyou Liu)
Chapter 5 DNA sequencing
(Dongyou Liu)
Chapter 6 DNA cloning vectors
(Laura C. García-Carnero, Uriel Ramírez-Sotelo, Ana P. Vargas-Macías, José A. Martínez-Álvarez, and Héctor M. Mora-Montes)
Chapter 7 DNA cloning
(Jake F. Watson, Sandra Arroyo-Urea, and Javier García-Nafría)
Chapter 8 DNA mutagenesis
(Dongyou Liu)
Chapter 9 CRISPR/Cas9-mediated genome editing: Current status
(Lei Tan, Fan Liao, Lei Lei, Deyong Duan, and Aibing Wang)
Chapter 10 DNA epigenetic modifications
(Kristen Abreu, Yuxiang Li, and Jian Feng)
Section II RNA Technologies
Chapter 11 RNA structure and function
(Dongyou Liu)
Chapter 12 RNA purification
(Ilgım Göktürk, Yeşeren Saylan, Fatma Yılmaz, and Adil Denizli)
Chapter 13 RNA amplification
(K. Rekha Devi, Archana Deka, and Kanwar Narain)
Chapter 14 In vitro RNA transcription
(Prashika Singh, Tiffany Smith, Patrick Arbuthnot, and Abdullah Ely)
Chapter 15 RNA sequencing
(Dongyou Liu)
Chapter 16 RNA cloning
(Dongyou Liu)
Chapter 17 RNA interference
(Anastasia M.W. Cooper, Kristopher Silver, and Kun Yan Zhu)
Chapter 18 RNA modifications
(Enakshi Sivasudhan, Zhiliang Lu, Jia Meng, and Rong Rong)
Section III Protein Technologies
Chapter 19 Protein structure and function
(Dongyou Liu)
Chapter 20 Protein purification
(Anupama Ghimire, Trần Thanh Tâm Phạm, Jeffrey R. Simmons, Qinyan Song, Anamika Sulekha, and Jan K. Rainey)
Chapter 21 Protein sequencing
(Dongyou Liu)
Chapter 22 Cell free gene expression
(Philip E. J. Smith, Jared L. Dopp, Nigel F. Reuel , and Javin P. Oza)
Chapter 23 Protein expression
(Justina Kazokaitė-Adomaitienė, and Patrick H. N. Celie)
Chapter 24 Polyclonal antibodies
(Masami Nakazawa, and Dongyou Liu)
Chapter 25 Monoclonal antibodies
(Andrew Mingee, Ana Luz Quiroga Campano, and Athanasios Mantalaris)
Chapter 26 Recombinant antibodies
(Ario de Marco)
Chapter 27 Protein modification
(Dongyou Liu)
Chapter 28 Protein-protein interactions: A technical overview
(Dongyou Liu)
Chapter 29 Protein-DNA interaction
(Giulio Ticli, Ornella Cazzalini, and Ennio Prosperi)
Chapter 30 Protein-RNA interaction
(Dongyou Liu)
Chapter 31 Protein-lipid interaction
(Dongyou Liu)
Chapter 32 Protein-carbohydrate interaction
(Dongyou Liu)
Section IV Molecular Approaches to Biotechnology
Diagnostics
Chapter 33 Molecular systematics
(Yan Zhang and Dongyou Liu)
Chapter 34 Nucleic acid diagnostics
(Juan García-Bernalt Diego, Pedro Fernández-Soto, and Antonio Muro)
Chapter 35 Immunodiagnostics
(Dongyou Liu)
Chapter 36 Mutation detection
(N. Anitha, and Dongyou Liu)
Chapter 37 Molecular epidemiology
(O.R. VinodhKumar, R. Karthikeyan, Rahul Kaushik, Ratan Kumar Choudhary, and Yashpal Singh Malik)
Chapter 38 Antimicrobial resistance: Causes, mechanisms, origins and characterization
(Aqsa Shahid, Aniqa Arshad, Bilal Aslam, Muhammad Akhtar Ali, Samman Munir, Sehar Aslam, and Mohsin Khurshid)
Chapter 39 Biofilms and their impact on human health: the beauty and the beast
(Veronica Georgiana Preda, Lauren Roberts, and Oana Săndulescu)
Chapter 40 Microbiome
(Inmaculada Perez-Prieto, Alberto Sola-Leyva, Nerea M. Molina, and Signe Altmäe)
Chapter 41 Genomics
(Galina N. Chelomina)
Chapter 42 Transcriptomics
(Dongyou Liu)
Chapter 43 Proteomics
(Vasileios Vrazas, and Katerina R. Katsani)
Chapter 44 Harnessing dynamic metabolomics for bioprocess prediction and beyond
(Guan Wang, Cees Haringa, Ju Chu, Yingping Zhuang, Wouter van Winden, and Henk Noorman)
Chapter 45 Biosensors
(Dongyou Liu)
Therapeutics
Chapter 46 Probiotics and prebiotics
(Roja Rajasekaran)
Chapter 47 Dietary supplements
(Si Qin)
Chapter 48 Vaccines
(Marinela Contreras, Weronika Grąźlewska, Paul D. Kasaija, and José de la Fuente)
Chapter 49 Phage therapy: A clinician’s guide
(Diana R. Alves, Katarzyna M. Danis-Wlodarczyk, and Stephen T. Abedon)
Chapter 50 Gene therapy
(Dongyou Liu)
Chapter 51 Stem cell therapy
(Shilpa Sharma, Madhan Jeyaraman, Sathish Muthu, and Rashmi Jain)
Chapter 52 Immuno- and targeted-therapies for cancer
(Raúl Barrera-Rodríguez, and Carlos David Pérez-Malagón)
Chapter 53 Biocontrol and bioremediation
(Xueyong Zhou, and Dongyou Liu)
Miscellaneous applications
Chapter 54 Forward and reverse genetics
(Dongyou Liu)
Chapter 55 Genetically modified bacteria: History, principle, and utility
(Ugur Azizoglu, Pratheesh Prakasam Thanka, Sudeepa Ennara Sudarsanan, Surya Sudheer, Bhuvnesh Prasad Sharma, and Ashok K. Rathoure)
Chapter 56 Genetically modified Caenorhabditis elegans as an experimental model
(Xun Suo, and Dongyou Liu)
Chapter 57 Genetically modified Drosophila melanogaster as an experimental model
(Masamitsu Yamaguchi, Hiroyuki Ida, and Hideki Yoshida)
Chapter 58 Genetically modified zebrafish as an experimental model
(Matthew R. Stoyek, Sara A. Rafferty, and T. Alexander Quinn)
Chapter 59 In vitro tissue engineering
(Loraine L.Y. Chiu, and Stephen D. Waldman)
Chapter 60 Transgenic animals
(Yi Hu, Xiaohong Xie, Lei Lei, Yuge Wang, Ning Xu, Kaiwen Xu, Deyong Duan, and Aibing Wang)
Chapter 61 Transgenic plants
(Hao Hu)
Chapter 62 Bioinformatics
(Yibi Chen, and Dongyou Liu)
INDEX
Biography
Dongyou Liu, Ph.D., studied veterinary science at Hunan Agricultural University, China and conducted postgraduate research on the generation and application of monoclonal antibodies for improved immunodiagnosis of human hydatidosis at The University of Melbourne, Australia. In the past three decades, he has worked at several research and clinical laboratories in Australia and the United States of America, with focuses on molecular characterization of microbial pathogens and detection of human genetic disorders and tumors/cancers. He is the first author of >50 original research and review articles in peer-reviewed international journals, the contributor of 294 book chapters, and the editor of Handbook of Listeria monocytogenes (2008), Handbook of Nucleic Acid Purification (2009), Molecular Detection of Foodborne Pathogens (2009), Molecular Detection of Human Viral Pathogens (2010), Molecular Detection of Human Bacterial Pathogens (2011), Molecular Detection of Human Fungal Pathogens (2011), Molecular Detection of Human Parasitic Pathogens (2012), Manual of Security Sensitive Microbes and Toxins (2014), Molecular Detection of Animal Viral Pathogens (2016), Laboratory Models for Foodborne Infections (2017), Pocket Guides to Biomedical Sciences: Tumors and Cancers – Central and Peripheral Nervous Systems (2017), Pocket Guides to Biomedical Sciences: Tumors and Cancers – Head, Neck, Heart, Lung and Gut (2017), Pocket Guides to Biomedical Sciences: Tumors and Cancers – Skin, Soft Tissue, Bone and Urogenitals (2017), Pocket Guides to Biomedical Sciences: Tumors and Cancers – Endocrine glands, Blood, Marrow and Lymph (2017), Handbook of Foodborne Diseases (2018), Handbook of Tumor Syndromes (2020), and Molecular Food Microbiology (2021), all of which are published by CRC Press. He is also a co-editor for Molecular Medical Microbiology, 2nd edition (2014), and Molecular Medical Microbiology, 3rd edition (2023), which are released by Elsevier.
