1st Edition
Methods in Cyanobacterial Research
This laboratory reference compiles the essential protocols used in cyanobacterial research. Cyanobacteria is a model system for biofuel production and sequestration of carbon dioxide to tackle global climate change due to increasing levels of greenhouse gases. Topics range from identification and growth parameters to biomolecule estimation and isolation. It also covers genome analysis and bioengineering of cyanobacteria for novel products.
Key Features:
- Provides step-by-step procedures and troubleshooting tips
- Covers identification, growth parameters and pigment estimation methodologies
- Describes isolation, characterization and purification of microcystin, phycobiliproteins and scytonemin from cyanobacteria
- Discusses genomics, proteomics and bioengineering of cyanobacteria from novel products
- Explains sample preparation and visualization protocol for electron microscopy-based analysis of cyanobacteria
This collection is useful to students and researchers in life sciences. It is also meant for industry experts who are involved in the production of biofuels, biofertilizers, other value-added products, and carbon sequestration using cyanobacteria.
Chapter 1: Evaluating the polyphasic approach in cyanobacterial taxonomy
Sagarika Pal, Naresh Kumar, Harsh Pant, Lira A. Gaysina and Prashant Singh
Chapter 2: Estimation of growth and photosynthetic pigments in cyanobacteria
Pankaj K. Maurya, Sapna Tiwari, Soumila Mondal, Anjali Gupta and Shailendra P. Singh
Chapter 3: Quantitative estimation of total carbohydrate, protein and lipid contents in cyanobacteria
Pankaj K. Maurya, Sapna Tiwari, Soumila Mondal, Anjali Gupta, Vinod Kumar and Shailendra P. Singh
Chapter 4: Isolation and purification of phycobiliproteins
Jyoti Jaiswal, Sonal Mishra, Neha Kumari, Varsha K. Singh and Rajeshwar P. Sinha
Chapter 5: Methods for estimation of oxidative stress indices in cyanobacteria
Vinod Kumar, Anjali Gupta, Soumila Mondal, Sapna Tiwari and Shailendra P. Singh
Chapter 6: Estimation of glutamine synthetase enzyme
Sonal Mishra, Neha Kumari, Varsha K. Singh and Rajeshwar P. Sinha
Chapter 7: Estimation of nitrate reductase enzyme
Sonal Mishra, Prashant R. Singh, Niharika Sahu and Rajeshwar P. Sinha
Chapter 8: Estimation of nitrogenase enzyme activity in cyanobacteria
Sonal Mishra, Amit Gupta, Ashish P. Singh and Rajeshwar P. Sinha
Chapter 9: Isolation and purification of cyanobacterial toxins
Sonal Mishra and Rajeshwar P. Sinha
Chapter 10: Isolation and purification of scytonemin
Neha Kumari, Sonal Mishra, Jyoti Jaiswal, Prashant R. Singh and Rajeshwar P. Sinha
Chapter 11: Isolation and purification of mycosporine-like amino acids
Sonal Mishra, Neha Kumari, Jyoti Jaiswal and Rajeshwar P. Sinha
Chapter 12: Protein estimation in cyanobacterial samples
Sonal Mishra, Jyoti Jaiswal and Rajeshwar P. Sinha
Chapter 13: Proteomic analysis of cyanobacteria employing 2-dimensional gel electrophoresis (2-DE)
Garvita Singh, Preeti Agarwal, Pritam Kaur, Akanksha Madan and Rajeshwar P. Sinha
Chapter 14: LC-MS/MS based proteomics study of cyanobacteria
Soumila Mondal, Subodh Kumar, Phulen Sarma, Bikash Medhi, Anjali Gupta, Sapna Tiwari, Pankaj K. Maurya and Shailendra P. Singh
Chapter 15: Monitoring photosynthetic performance in cyanobacteria by a modulated fluorometer
Sapna Tiwari, Pankaj K. Maurya, Soumila Mondal, Anjali Gupta, Donat-P. Häder and Shailendra P. Singh
Chapter 16: Reactive oxygen species measurement in cyanobacteria using 2′,7′- dichlorodihydrofluorescein diacetate (DCFH-DA) dye and fluorescence microscope or flow cytometer
Soumila Mondal, Anjali Gupta, Sapna Tiwari, Pankaj K. Maurya, Priyul Pandey and Shailendra P. Singh
Chapter 17: Flow cytometry-based methods for estimating DNA content and live-dead cells in cyanobacteria
Soumila Mondal, Anjali Gupta, Sapna Tiwari, Rinkesh Gupta and Shailendra P. Singh
Chapter 18: Genomic DNA and RNA extraction from cyanobacteria
Anjali Gupta, Soumila Mondal, Sapna Tiwari, Donat-P. Häder, Rajeshwar P. Sinha and Shailendra P. Singh
Chapter 19: Detection and quantification of DNA damage in cyanobacteria by immunodot- blot assay
Prashant R. Singh, Jainendra Pathak, Rajneesh, Deepak Kumar, Amit Gupta, Ashish P. Singh, Varsha K. Singh and Rajeshwar P. Sinha
Chapter 20: Engineering cyanobacteria for the synthesis of novel products
Rungaroon Waditee-Sirisattha and Hakuto Kageyama
Chapter 21: Sample preparation and visualization protocol for electron microscopy-based analysis of cyanobacteria
Soumila Mondal, Bhaskar Sharma, Abhisek Majumdar, Sapna Tiwari, Pankaj K. Maurya, Anjali Gupta, Shailendra P. Singh and Prabhakar Singh
Chapter 22: Cyanobacterial biofertilizer production technology
Sonal Mishra and Rajeshwar P. Sinha
Chapter 23: Biomass composition measurement and intracellular flux analyses for insights into cyanobacterial metabolism
Piyush Pachauri, Preeti Rai and Shireesh Srivastava
Chapter 24: In silico genome assembly and functional annotation
Pannaga P. Jutur, Ashish Kumar, Mohammed Rehmanji, Rabinder Singh, Asha A. Nesamma
Index
Biography
Dr. Shailendra P. Singh is an Assistant Professor at the Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University (BHU), Varanasi, India. He earned his PhD from the Department of Biology, Friedrich-Alexander University, Erlangen-Nuremberg, Germany.
Prof. Dr. Rajeshwar P. Sinha, DAAD Fellowship Awardee and Fellow, Society for Applied Biotechnology, India, is a Professor of Molecular Biology, Centre of Advanced Study in Botany, Institute of Science, Banaras Hindu University (BHU), Varanasi, India.
Prof. Donat-P. Häder is the past-Director of the Botanical Institute and held the chair of Ecophysiology of Plants at the Friedrich-Alexander University, Erlangen-Nürnberg, Germany. He was also the director of the Botanical Garden.
